ICS of HZAU

Dr. WenWu Guo

Education

1993 – 1998 Ph.D. Huazhong Agricultural University, Fruit Science, Wuhan, China

1989 – 1993 B.A. Huazhong Agricultural University, Horticulture / Fruit Science, Wuhan, China

Professional Experience

2009.3 – 4 Senior visiting fellow in Kagoshima University, Japan (Supported by JSPS)

2007.3 – 5 Senior visiting fellow in Dept of Horticulture, Australian CSIRO Plant Industry

2004 – present Professor, Huazhong Agricultural University

2001 – 2003 Postdoctoral research in Citrus REC, University of Florida, USA

2000 – 2003 Associate professor, Huazhong Agricultural University

1998 – 1999 Assistant professor, Huazhong Agricultural University

Services for academic communities

Co-Editor-in-Chief: Scientia Horticulturae

Associate editors: Plant Cell Tissue & Organ Culture; Tree Genetics & Genomes

Program emphasis

Cell engineering and citrus genetic improvement. 1) Germplasm enhancement by cell engineering: protoplast fusion to produce somatic hybrids / cybrids, doubled diploid (tetraploid) exploitation, ploidy crossing & embryo rescue for seedlesstriploid breeding, and anther culture to generate haploid / dihaploid lines; 2) Molecular dissection of specific traits: omics-based approaches to reveal genetics / epigenetics of citrus polyploids, and to exploit male sterility / seedlessness related genes using novel male sterile cybrid lines; protoplast regeneration based gene editing and molecular breeding.

• 1. Tan FQ, Zhang M, Xie KD, Fan YJ, Song X, Wang R, Wu XM, Zhang HY, Guo WW* (2019) Polyploidy remodels fruit metabolism by modifying carbon source utilization and metabolic flux in Ponkan mandarin. Plant Science 289:110276

• 2. Long JM, Liu CY, Feng MQ, Liu Y, Wu XM*, Guo WW* (2018) miR156-SPLs module regulates somatic embryogenesis induction in citrus callus. Journal of Experimental Botany 69: 2979-2993

• 3. Tan FQ, Tu H, Wang R, Wu XM, Xie KD, Chen JJ, Zhang HY, Xu J, Guo WW* (2017) Metabolic adaptation following genome doubling in citrus doubled diploids revealed by non-targeted metabolomics. Metabolomics 13: 143

• 4. Long JM, Liu Z, Wu XM*, Fang YN, Jia HH, Xie ZZ, Deng XX, Guo WW* (2016) Genome-scale mRNA and small RNA transcriptomic insights into initiation of citrus apomixis. Journal of Experimental Botany 67: 5743–5756

• 5. Fang YN, Zheng BB, Wang L, Yang W, Wu XM, Xu Q, Guo WW* (2016) High-throughput sequencing and degradome analysis reveal altered expression of miRNAs and their targets in a male-sterile cybrid pummelo (Citrus grandis). BMC Genomics 17:591

• 6. Guo WW*, Liang WJ, Xie KD, Xia QM, Fu J, Guo DY, Xie ZZ, Wu XM, Xu Q, Yi HL, Deng XX (2016) Exploitation of polyploids from 39 citrus seedling populations. Acta Horticulturae 1135: 11-16

• 7. Tan FQ, Tu H, Liang WJ, Long JM, Wu XM, Zhang HY, Guo WW* (2015) Comparative metabolic and transcriptional analysis of a doubled diploid and its diploid citrus rootstock (C. junos cv. Ziyang xiangcheng) suggests its potential value for stress resistance improvement. BMC Plant Biology 15: 89

• 8. Wang SM, Lan H, Cao HB, Xu Q, Chen CL, Deng XX, Guo WW* (2015) Recovery and characterization of homozygous lines from two sweet orange cultivars via anther culture. Plant Cell Tissue Organ Culture 123:633-644

• 9. Wu XM, Kou SJ, Liu YL, Fang YN, Xu Q, Guo WW* (2015) Genome-wide analysis of small RNAs in non-embryogenic and embryogenic tissues of citrus: microRNA and siRNA mediated transcript cleavage involved in somatic embryogenesis. Plant Biotechnology Journal 13: 383-394

• 10. Xie KD, Xia QM, Wang XP, Liang WJ, Wu XM, Grosser JW, Guo WW* (2015) Cytogenetic and SSR marker evidence of mixed disomic, tetrasomic and intermediate inheritance in a citrus allotetraploid somatic hybrid between ‘Nova’ tangelo and ‘HB’ pummelo. Tree Genetics Genomes 11: 112

• 11. Fang YN, Qiu WM, Wang Y, Wu XM, Xu Q, Guo WW* (2014) Identification of differentially expressed microRNAs from a male sterile Ponkan mandarin (Citrus reticulata Blanco) and its fertile wild type by small RNA and degradome sequencing. Tree Genetics Genomes 10: 1567-1581

• 12. Xiao SX, Biswas MK, Li MY, Deng XX, Xu Q, Guo WW* (2014) Production and molecular characterization of diploid and tetraploid somatic cybrid plants between male sterile Satsuma mandarin and seedy sweet orange cultivars. Plant Cell Tissue Organ Culture 116: 81-88

• 13. Xie KD, Wang XP, Biswas MK, Liang WJ, Xu Q, Grosser JW, Guo WW* (2014) 2n megagametophyte formed via SDR contributes to tetraploidization in polyembryonic ‘Nadorcott’ tangor crossed by citrus allotetraploids. Plant Cell Reports 33:1641–1650

• 14. Zheng BB, Fang YN, Pan ZY, Sun L, Deng XX, Grosser JW, Guo WW* (2014) iTRAQ based quantitative proteomics analysis revealed alterations of carbohydrate metabolism pathways and mitochondrial proteins in a male sterile cybrid pummelo. Journal of Proteome Research 13: 2998-3015

• 15. Ahmad T, Sablok G, Tatarinova T, Xu Q, Deng XX, Guo WW* (2013) Evaluation of codon biology in Citrus and Poncirus trifoliata based on genomic features and frame corrected expressed sequence tags. DNA Research 20: 135-150

• 16. Guo WW*, Xiao SX, Deng XX (2013) Somatic cybrid production via protoplast fusion for citrus improvement. Scientia Horticulturae 163: 20-26

• 17. Ge XX, Chai LJ, Liu Z, Wu XM, Deng XX, Guo WW* (2012) Transcriptional profiling of genes involved in embryogenic, non-embryogenic calluses and somatic embryogenesis of Valencia sweet orange by SSH based microarray. Planta 236: 1107-1124

• 18. Kou SJ, Wu XM, Liu Z, Liu YL, Xu Q, Guo WW* (2012) Selection and validation of suitable reference genes for miRNA expression normalization by quantitative RT-PCR in citrus somatic embryogenic and adult tissues. Plant Cell Reports 31: 2151-2163

• 19. Qiu WM, Zhu AD, Wang Y, Ge XX, Chai LJ, Deng XX, Guo WW* (2012) Comparative transcript profiling of gene expression between seedless Ponkan mandarin and its seedy wild type during floral organ development by suppression subtractive hybridization and cDNA microarray. BMC Genomics 13: 397

• 20. Xu SX, Cai XD, Tan B, Li DL, Guo WW* (2011) Effect of ploidy increase on transgene expression: example from Citrus diploid cybrid and allotetraploid somatic hybrid expressing the EGFP gene. Protoplasma 248: 531-540

• 21. Wu XM, Liu MY, Ge XX, Xu Q, Guo WW* (2011) Stage and tissue-specific modulation of ten conserved miRNAs and their targets during somatic embryogenesis of Valencia sweet orange. Planta 233: 495-505

• 22. Tan B, Li DL, Xu SX, Fan GE, Fan J, Guo WW* (2009) Highly efficient transformation of GFP and KNU genes into precocious trifoliate orange (Poncirus trifoliata [L.] Raf), a potential model genotype for functional genomics studies in Citrus. Tree Genetics Genomes 5: 529-537

• 23. Cai XD, Fu J, Deng XX, Guo WW* (2007) Production and molecular characterization of potential seedless cybrid plants between pollen sterile Satsuma and two seedy Citrus cultivars. Plant Cell Tissue Organ Culture 90: 275-283

• 24. Guo WW*, Grosser JW* (2005) Somatic hybrid vigor in Citrus: direct evidence from protoplast fusion of an embryogenic callus line with a transgenic mesophyll parent expressing the GFP gene. Plant Science 168: 1541-1545

• 25. Guo WW, Duan YX, Olivares-Fuster O, Wu ZC, Arias CR, Burns JK, Grosser JW (2005) Protoplast transformation and regeneration of transgenic Valencia sweet orange plants containing a juice quality related pectin methylesterase gene. Plant Cell Reports 24: 482-486

• 26. Guo WW, Prasad D, Cheng YJ, Serrano P, Deng XX, Grosser JW (2004) Targeted cybridization in Citrus: transfer of Satsuma cytoplasm to seedy cultivars for potential seedlessness. Plant Cell Reports 22: 752-758

• 27. Guo WW, Cheng YJ, Deng XX (2002) Regeneration and molecular characterization of intergeneric somatic hybrids between Citrus reticulata and Poncirus trifoliata. Plant Cell Reports 20: 829-834

• 28. Guo WW, Deng XX (1999) Intertribal hexaploid hybrid plants regeneration from electrofusion between diploids of Citrus sinensis and its sexually incompatible relative, Clausena lansium. Theor Appl Genet 98: 581-585

• 29. Guo WW, Deng XX (1998) Somatic hybrid plantlets regeneration between Citrus and its wild relative, Murraya paniculata via protoplast electrofusion. Plant Cell Reports 18: 297-300