ICS of HZAU

Dr. HongYu Zhang

Education

1995–1998 Ph.D. in insecticidal microbiology Huazhong Agricultural University, , Wuhan, China

1988-1991 Master of Science in Entomology Huazhong Agricultural University ， Wuhan, China

1982–1986 Bachelor of Science in Plant Protection Hunan Agricultural University, Changsha, China

Professional Experience

2004.8-present Professor, Huazhong Agricultural University

2013.8–2014.2 Senior visiting scholar in University of Florida, USA

2005.5-2005.8 Senior visiting scholar in Michigan State University, USA

2002.9-2004.9 Postdoctoral Research Fellow, AgResearch Lincoln, New Zealand

2001.7-2002.8 Postdoctoral Research Fellow, Department of Biology, Konstanz University, Germany

1999.2- 1999.12 Visiting Research Fellow, Agricultural Research Center, Israel

1998.7- 2004.8 Associate professor, Department of Plant Protection, Huazhong Agricultural University

Services for academic communities

Scientific advisor of International Foundation for Science (IFS, Sweden)

Editorial board members of Asian Journal of Plant Sciences and Hubei Plant Protection

Council Member of theChinese Cereals and Oils Association

Specialized Committee Member of Horticultural Pest Control, China Society of Plant Protection

Specialized Committee Member of Insecticide and Toxicology, The Entomological Society of China

Specialized Committee Member of Urban Entomology, The Entomological Society of China

Program emphasis

1. Insect Molecular Biology and Microbiology

(1) Molecular Mechanism of Developmental, Reproduction and Behavior

Study funcations of the SPR, TRA, ORCO, NOA, RAB11 etc key genes and miRNA in the sex differentiation and host lacation of citrus insects (fruit fly) using our RNAi and CRISPR-Cas9 technology platform, to develope SIT without Radiation using silencing sex determination genes.

(2) Interaction between insects and gut symbiotic bacteria

Gut symbiosis is very important to host insect behavious, We have uncovered molecular diversity, community composition and physiological function of the fruit fly and scarab gut microbes; clarify the molecular mechanism of BdDuox regulates gut bacterial communities homeostasis of Bactrocera dorsalis.

(3) The molecular mechanism of microRNA regulating in insect spermatogenesis

By using small RNA high-throughput sequencing technology, we studied the comparative profile of the miRNA transcriptome in different development B. dorsalis testes and identified miRNAs involved in the spermatogenesis; analyzed the role of miR-8-3p in the spermatogenesis and found that miR-8-3p regulate mitoferrin expression to sustain the normal transportation of iron ion. The current study of miRNAs improves the understanding of molecular mechanisms of insect spermatogenesis and develops non-radiated and non-transgenic SIT techniques to control B. dorsalis based on the key miRNAs involved in the spermatogenesis.

2. Early warning and expert systems for Citrus Insect: Based on 3S 3S Technology, constructing data bases for Citrus Insect including Fly Early Warning and Forecasting System Database、Information Database for Citrus Insect、Expert system for Citrus Insects and so on, and set up a web-real time Early warning and expert systems for Citrus Insect: http://cpis.hzau.edu.cn/.

3. Biology and ecology, Green Pests Control Techniques: (1) Biology and ecology of Fruit fly Bactrocera minax and Asian citrus psyllid; Effect of different ground cover management on spider mites and their phytoseiid enemies in citrus orchards; Functional and Numerical Responses of Three Species of Predatory Phytoseiid Mites to Thrips flavidulus and so on. (2) Developing the sex pheromones and high efficient baits of Bactrocera minax, and its field application technology. (3) Pathogenic microorganism resources investigation, fermentation, formulation and developing microbial pesticide and application technique in the field : Aschersonia placenta of white fly，Isaria fumosorosea, Hirsutella Citriformis of Asian citrus psyllid. (4) Mass rearing and application techniques of the predatory mites enemy: Amblyseius eharai etc. (5) Developing the physical trap baits of thrip and weevil.

• 1. Yao ZC, Wang AL, Li YS, Cai ZH, Lemaitre B, Zhang HY* 2015. The dual oxidase gene BdDuox regulates the intestinal bacterial community homeostasis of Bactrocera dorsalis. ISME J. doi:10.1038/ismej.2015.202

• 2. Tariq K., Metzendorf C., Peng W., Sohail S., Zhang HY*. 2016. miR-8-3p regulates mitoferrin in the testes of Bactrocera dorsalis to ensure normal spermatogenesis. Scientific RepoRts | 6:22565 | DOI: 10.1038/srep22565

• 3. Zheng W.W.* , Luo D.Y., Wu F.Y., Wang J.L., Zhang H.Y.*. 2016. RNA sequencing to characterize transcriptional changes of sexual maturation and mating in the female oriental fruit fly Bactrocera dorsalis. BMC Genomics (2016) 17:194 DOI 10.1186/s12864-016-2532-6

• 4. Wang S.Q., Zhang H.Y.*, Li Z.L. 2016. Small-Scale Spatio-Temporal Distribution of Bactrocera minax (Enderlein) (Diptera: Tephritidae) Using Probability Kriging. Neotrop Entomol DOI 10.1007/s13744-016-0384-z

• 5. Li, X.X., Dong, X.L., Zou, C., Zhang, H.Y.*. 2015. Endocytic pathway mediates refractoriness of insect Bactrocera dorsalis to RNA interference. Scientific reports. 5:8700

• 6. Tariq K., Peng W., Saccone G., Zhang H.Y.*. 2015. Identification, characterization and target gene analysis of testicular microRNAs in the oriental fruit fly Bactrocera dorsalis. Insect Molecular Biology.doi: 10.1111/imb.12196

• 7. Peng W., Zheng W.P., Alfred M. Handler, Zhang H.Y.*. 2015. The role of the transformer gene in sex determination and reproduction in the tephritid fruit fly, Bactrocera dorsalis (Hendel).Genetica. 143:717–727

• 8. Tariq K., Noor M. ,Saeed S., Zhang H.Y.*. 2015. The Effect of Ultraviolet-A Radiation Exposure on the Reproductive Ability, Longevity, and Development of the Dialeurodes citri (Homoptera: Aleyrodidae) F1 Generation. Environ. Entomol. 1–5 (2015); DOI: 10.1093/ee/nvv133

• 9. Zheng W.P., Liu Y.R., Zheng W.W., Xiao Y.L., Zhang H.Y.*. 2015. Influence of the silencing sex-peptide receptor on Bactrocera dorsalis adults and offspring by feeding with ds-spr. Journal of Asia-Pacific Entomology. 18: 477–481

• 10. Dong X. L., Li Q.J., Zhang H.Y.*. 2015. The noa gene is functionally linked to the activation of the Toll/Imd signaling pathways in Bactrocera dorsalis (Hendel). Developmental and Comparative Immunology. DOI: 10.1016/j.dci.2015.09.009

• 11. Chen A.E., Zheng W.W., Zheng W.P., Zhang H.Y.*. 2015. The effects of RNA interference targeting Bactrocera dorsalis ds-Bdrpl19 on the gene expression of rpl19 in non-target insects. Ecotoxicology. 24:595–603

• 12. Sheng P., Li Y.S., Marshall Sean D.G.and Zhang H.Y.*. 2015. High Genetic Diversity of Microbial Cellulase and Hemicellulase Genes in the Hindgut of Holotrichia parallela Larvae.Int. J. Mol. Sci. 16: 16545-16559

• 13. Marco S. mini*, Kallare P. A., Javaregowda N., Zhang H.Y.*, Weiwei Zheng, Giuseppe Saccone1. 2014. De Novo Assembly and Transcriptome Analysis of the Mediterranean Fruit Fly Ceratitis capitata Early Embryos. PLOS ONE 9(12):e114191

• 14. Giuseppe S.*, Christos L., Zhang H.Y.*. Valeria P., Manuela Di N. , Maria P., Marco S. mini. 2014. Male-specific phosphorylated SR proteins in adult flies of the Mediterranean Fruitfly Ceratitis capitata. BMC Genetics 15 (Suppl 2):S6

• 15. Yao H. F., Zheng W. W., Tariq K., Zhang H.Y.*. 2014. Functional and Numerical Responses of Three Species of Predatory Phytoseiid Mites (Acari: Phytoseiidae) to Thrips flavidulus (Thysanoptera: Thripidae) Neotrop Entomol. 43:437–445

• 16. Wang A L,Yao Z C,Zheng W W,Zhang H.Y.*.2014. Bacterial Communities in the Gut and Reproductive Organs of Bactrocera minax (Diptera:Tephritidae) Based on 454 Pyrosequencing.PLoS ONE9(9): e106988. doi:10.1371/journal.pone.0106988

• 17. Wang H.X., Jin L. Peng T., Zhang H.Y.*, Chen Q.L. and Hua Y.J. 2014.Identification of cultivable bacteria in the intestinal tract of Bactrocera dorsalis from three different populations and determination of their attractive potential. Pest Manag Sci, 70:80–87

• 18. Sheng P., Xu J., Saccone G., Li K.B., Zhang HY*. 2014. Discovery and characterization of endo-xylanase and β-xylosidase from a highly xylanolytic bacterium in the hindgut of Holotrichia parallela larvae. J Mol Catal B-Enzym 105, 33-40

• 19. Zhang B., Zheng W.W., Zhao W.J., Xu X.N., Liu J. & Zhang H.Y. *. 2013.Intraguild predation among the predatory mites Amblyseius eharai, Amblyseius cucumeris and Amblyseius barkeri, Biocontrol Science and Technology, 24:1, 103-115

• 20. Chen H. L., Zhang H.Y.*, Throne J.E. and Zhu K.Y. 2014. Transcript analysis and expression profiling of three heat shock protein 70 genes in the ectoparasitoid Habrobracon hebetor (Hymenoptera: Braconidae). Insect Science. 21, 415–428, doi:10.1111/1744-7917.12032

• 21. Zhao W. J., Zheng W. W. *, Zhang B., Yu G. L., Hu S. Q., Xuenong Xu X. N., Zhang H.Y. *, 2014. Effect of different ground cover management on spider mites (Acari: Tetranychidae) and their phytoseiid (Acari: Phytoseiidae) enemies in citrus orchards. Biocontrol Science and Technology. 24, 6:705–709

• 22. Wang P.P., Song X.H., Zhang H.Y.*. 2013. High genetic variation among Aschersonia placenta (Clavicipitaceae) isolates from citrus orchards in China，Genetics and Molecular Research 12 (4): 6192-6202

• 23. Huang S.W., Zhang H.Y.*.2013. Impact of Environmental Heterogeneity and Life stage on the Hindgut Microbiota of Holotrichia parallela larvae ( Coleoptera: Scarabaeidae). PLoS ONE 8(2): e57169. doi:10.1371/journal.pone.0057169

• 24. Zheng W.W. ,Peng W.,Zhu C.P.,Zhang Q., Saccone G.and Zhang H.Y.*.2013 Identification and Expression Profile Analysis of Odorant Binding Proteins in the Oriental Fruit Fly Bactrocera dorsalis. International Journal of Molecular Sciences 2013, 14, 14936-14949

• 25. Wang P.P., Song X.H., Zhang H.Y.*. 2013. Isolation and characterization of Aschersonia placentafrom citrus orchards and its pathogenicity towards Dialeurodes citri(Ashmead). Journal of Invertebrate Pathology 112:122–128

• 26. Chen H.L., Zhang H.Y.*. Zhu k.y., Throne J.*. 2013. Performance of diapausing parasitoid wasps, Habrobracon hebetor, after cold storage. Biological Control 64: 86–194

• 27. Zheng, W.W., Peng, T., He W., Zhang H.Y.*, 2012. High-Throughput Sequencing to Reveal Genes Involved in Reproduction and Development in Bactrocera dorsalis (Diptera: Tephritidae). PLoS ONE 7(5): e36463

• 28. Zheng W. W., Zhu Ch P., Peng T., Zhang H.Y.*.2012. Odorant receptor co-receptor Orcois upregulated by methyl eugenol in male Bactrocera dorsalis (Diptera: Tephritidae). Journal of Insect Physiology 58:1122–1127

• 29. Chen, H.L., Zhang,H.Y.*, Zhu, K.Y. and Throne J.E.. 2012. Induction of reproductive diapause in Habrobracon hebetor (Hymenoptera: Braconidae) when reared at different photoperiods at low temperatures. Environmental Entomology 41: 697-705

• 30. Huang, S.W., Sheng, P., Zhang, H.Y.*, 2012. Isolation and identification of cellulolytic bacteria from the gut of Holotrichia parallela larvae (Coleoptera: Scarabaeidae). International Journal of Molecular Sciences 13: 2563-2577