ICS of HZAU

Dr. Fang Ding

EDUCATION

09/1997-06/2001 B.Sc. Plant Protection department, Huazhong Agricultural University, Wuhan, 430070, Hubei P. R. China

09/2001-06/2006 M.Sc and Ph.D. College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei P. R. China ( Supervisor: Guoping Wang).

Fruit Tree Research Institute, Guangdong academy of Agricultural Science, Guangzhou, 510640, Guangdong, P. R. China ( Vice supervisor: Ganjun Yi).

Ph. D thesis: Research on Molecular Characteristic of Citrus Huanglongbing Pathogen and Viruses Mix-infected and the Elimination by Cryopreservation Protocol.

PROFESSIONAL EXPERIENCE

10/2015- Present Associate professor. College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.

Permanent staff of the National Key Laboratory of Agromicrobiology and National Indoor Conservation Center of Virus-free Germplasm of Fruit Crops.

10/2012-10/2015 Visiting Scientist (Supervisor: Dr. John. S. Hartung). Molecular Plant Pathology Laboratory. USDA-ARS (MD).

Research projects: Serological detection and identification of HLB bacteria from citrus.

01/2011-09/2012 Research Scholar (Supervisor: Dr. Yongping Duan). U.S. Horticultural Research Laboratory. USDA-ARS (FL). Research

projects: Response of prophages in Ca. Liberibacter under stress conditions.

01/2009-12/2010 Associate professor. College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.

07/2006-12/2008 Lecture. College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.

PROFESSIONAL SKILLS

Plant Pathology: Mycology, bacteriology, virology, disease diagnosis, microbial ecology, disease assessment, ELISA, DTBIA, microscopy, Fluorescence microscopy

Molecular Biology: DNA, RNA, protein extraction, PCR, quantitative RT-PCR, AFLP, DNA cloning, DNA sequencing, Southern and Northern blots, vector construction, insertion mutagenesis, SDS-PAGE.

Computer Software: Microsoft Word, Excel, PowerPoint, Signal P, DNAman, DNA Star, and statistics software SAS etc.

GRANTS AWARDED

Fundamental Research Funds for the Central Universities, Research on the fast detection of Candidatus Liberibacter asiaticus by Immune colloidal gold technique (2662016PY099). Chief Investigator.

National Natural Science Foundation of China, Isolation of Resistance Related-genes to Ca. Liberibacter asiaticus with SSH in the Murraya paniculata (No.30700550). Chief Investigator.

Research on high throughput molecular detection techniques of citrus Huanglongbing. Science Foundation for the Excellent Youth Scholars of Ministry of Education of China (20070504079). Chief Investigator.

Screening and cloning of Resistance Related-genes to Candidatus Liberibacter asiaticus by SSH in the trifoliate orange. International Foundation for Science (IFS: D/4460-1). Chief Investigator.

Natural Science Foundation of Hubei province. Establishment of rapid detection system for citrus virus agents (2006AA203B05). Attendee.

National 863 of China. Research on high throughput molecular detection techniques of important plant quarantine disease for open port (No. 2006AA10Z434). Attendee.

Research on the integrated management of citrus Huanglongbing and citrus canker in China. (No. 201003067). Attendee.

AWARDS WINNING

The 2nd Award of Science and Technology Progress by Hubei Province (No.71523086, 3rd Accomplisher).

The 3rd Award in 12th Hubei best essay award in Natural Sciences.

Excellent Headteacher of Huazhong Agricultural University (2007-2008).

Excellent doctorial dissertation of Huazhong Agricultural University (2006).

PROFESSIONAL ACTIVITIES AND SERVICES

Journal Referee for

International: Scientia Horticulturae; Agricultural Science Research Journal;

China: Molecular Plant Breeding; Fujian Journal of Agricultural Sciences

Professional Affiliation

2010-present American Phytopathological Society Member

2006-present Chinese Society of Plant Pathology

• 1. Ding Fang, Duan Yongping, Yuan Qing, Shao Jonathan and John S. Hartung. Serological detection of ‘Candidatus Liberibacter asiaticus’ in citrus, and identification by GeLC-MS/MS of a chaperone protein responding to cellular pathogens. Scientific Reports | 6:29272 | DOI: 10.1038/srep29272

• 2. Ding F, Duan Y, Paul C, Brlansky RH, Hartung JS (2015) Localization and Distribution of 'Candidatus Liberibacter asiaticus’ in Citrus and Periwinkle by Direct Tissue Blot Immuno Assay with an Anti-OmpA Polyclonal Antibody. PLoS ONE 10(5): e0123939. doi:10.1371/journal.pone.0123939

• 3. Fang Ding, Cristina Paul, Ronald H. Brlansky and John S. Hartung. Direct Tissue Blot Immuno Assay and Immune Capture-PCR for Diagnosis and Detection of ‘Candidatus Liberibacter asiaticus’. Scientific Reports. In review.

• 4. Fang Ding, Shouan Zhang and Yongping Duan. Response of two prophages in 'Candidatus Liberibacter asiaticus’ the unculturable bacteria under stress conditions. Scientific Reports. In Preparation.

• 5. Ding F, Duan Y, Paul C, Brlansky RH, Hartung JS (2015) Localization and Distribution of 'Candidatus Liberibacter asiaticus’ in Citrus and Periwinkle by Direct Tissue Blot Immuno Assay with an Anti-OmpA Polyclonal Antibody. PLoS ONE 10(5): e0123939. doi:10.1371/journal.pone.0123939

• 6. Guixia Hao, Marco Pitino, Fang Ding, Hong Lin, Ed Stover and Yongping Duan. Induction of innate immune responses by flagellin from the intracellular bacterium, ‘Candidatus Liberibacter solanacearum’. BMC Plant Biology 2014, 14: 211. doi:10.1186/s12870-014-0211-9

• 7. Hoffman MT, Doud MS, Williams L, Zhang MQ, Ding F, Stover E, Hall D, Zhang S, Jones L, Gooch M, Fleites L, Dixon W, Gabriel D, Duan YP. Heat treatment eliminates 'Candidatus Liberibacter asiaticus' from infected citrus trees under controlled conditions. Phytopathology. 2013, 103(1):15-22. doi: 10.1094/PHYTO-06-12-0138-R.

• 8. Zhang Y, Yin J, Jiang D, Xin Y, Ding F, et al. A Universal Oligonucleotide Microarray with a Minimal Number of Probes for the Detection and Identification of Viroids at the Genus Level. PLoS ONE 2013, 8(5): e64474.

• 9. Fang Ding, Xiuxin Deng, Ni Hong, Yun Zhong, Ganjun Yi and Guoping Wang,. 2009. Phylogenetic Analysis of Citrus Huanglongbing Bacterium based on the Sequences of 16S rDNA and 16S rDNA/23S rDNA Intergenic Regions among isolates in China. European Journal of Plant Pathology, 124: 495-503

• 10. Fang Ding, Shuangxia Jin, Ni Hong, Yun Zhong, Qing Cao, Ganjun Yi and Guoping Wang. 2008. Vitrification–cryopreservation, an efficient method for eliminating Candidatus Liberibacter asiaticus, the citrus Huanglongbing pathogen, from in vitro adult shoot tips. Plant Cell Reports, 27(2), 241-250

• 11. F. Ding, G. Wang, G. Yi, Y. Zhong, J. Zeng and B. Zhou.Infection of wampee and lemon by citrus Huanglongbing pathogen (Candidatus Liberibacter Asiatic us) in China. Journal of Plant Pathology, 2005,87(3),207-212

• 12. Fang Ding, Hong Ni, Zhong Yun, Yi Ganjun, and Wang Guoping. 2008. Cloning and analyzing of 5’A and F’ variation sequences of Citrus tristeza virus genomic RNA of isolates from different regions. Acta Phytopathologica Sinica 38 (3): 252-257

• 13. Fang Ding, Hong Ni, Zhong Yun, Yi Ganjun, and Wang Guoping. 2008. Studies on 16S rDNA Sequence of Citrus Huanglongbing Bacteria in China. Acta Horticulturae Sinica. 35 (5) : 649 - 654

• 14. Ding Fang, Wang Guoping, Yi Ganjun, Hong Ni, and Zhong Yun. 2007. Comparison of detection sensitivity of different primer pairs for citrus huanglongbing pathogen. Acta phytophylacia Sinica, 34(4), 364-368

• 15. Fang Ding, Yi Ganjun, Wang Guoping, Cao Qing, Hong Ni, and Zhong Yun. 2007. Cloning and analyzing a full length cDNA of Citrus exocortis viroid YC isolate from Guang Dong. Molecular Plant Breeding.5(6):871-874

• 16. Fang Ding, Qing Cao, Guoping Wang, Ganjun Yi, and Yun Zhong. 2006, Studies on the Simultaneous Detection of Citrus Huanglongbing Pathogen, Citrus exocortis viroid, Citrus tristeza virus by Multiplex RT-PCR. Acta Horticulturae Sinica. 33 (5) : 947-952

• 17. Xiao Yuanhui; Ding Fang; Zeng Jiwu; Yi Ganjun; Zhang Qiuming. 2006, Simultaneous detection of Citrus Huanglongbing and Citrus tristeza virus by multiplex RT-PCR. Journal of Fruit Science. 23( 4) : 642-645

• 18. Fang Ding, Guoping Wang, Ganjun Yi, Yun Zhong. 2005, Infection of Wampee and Lemon by Citrus Huanglongbing pathogen (Candidatus Liberibacter asiaticum) in China. Journal of Plant Pathology, 87(3):207-212

• 19. Fang Ding, Guoping Wang, Ganjun Yi. 2004, Research on the PCR and Nested-PCR Detection of Citrus Huanglongbing Pathogen. Acta Horticulturae Sinica, 31 (6) : 803-806

• 20. Fang Ding, Guoping Wang, Ganjun Yi. 2004, PCR detection of Citrus Huanglongbing pathogen. Horticulturae of Guangdong. 5 (1)：60-62

• 21. Fang Ding, Guoping Wang, Ganjun Yi. 2003, Advances on citrus Huanglongbing research. Horticulturae of Guangdong. 4 (4) :17-18