ICS of HZAU

郭文武

郭文武简历:男,博士、教授、博士生导师。1993年7月本科毕业于华中农业大学园艺系;1993-1998年,华中农业大学攻读硕士、博士学位;1998年6月毕业,获博士学位并留校任教。1999、2003年,分别晋升为副教授、教授。2001-2003年,美国佛罗里达大学柑橘研究与教育中心博士后合作研究。入选国家杰出青年科学基金、教育部长江学者特聘教授、中组部万人计划第一批科技创新领军人才、农业部全国农业科研杰出人才、科技部首批中青年科技创新领军人才、教育部首批新世纪优秀人才;牵头《园艺植物细胞工程与种质创新》教育部创新团队和湖北省创新群体、《柑橘细胞工程与遗传改良》农业部创新团队;曾获国家科技进步二等奖(排名第3)、中国青年科技奖。受聘国务院学位委员会第7届学科评议组(园艺学)成员,国际SCI刊物《Scientia Horticulturae》共同主编,《Plant Cell Tissue Organ Culture》、《Tree Genetics Genomes》Associate Editor;中国园艺学会分子育种分会副理事长,中国柑橘学会理事、中国细胞生物学学会理事。先后主持国家自然科学基金(包括杰青、重点)、科技部973计划课题、863计划课题、教育部项目、农业部项目、湖北省自然科学基金(杰青、重点、创新群体)、瑞典国际科学基金等30余项。



联系方式 :

Tel: 027-87281543 (O)

Email: guoww@mail.hzau.edu.cn

研究方向:

柑橘细胞工程与遗传改良

重点内容 :

针对我国柑橘在品种、品质等方面存在的问题,以及常规育种中遇到的珠心胚干扰等生殖障碍,借助现代分子标记和组学手段,重点开展细胞工程与遗传改良研究。旨在提高育种效率、缩短育种周期,培育具有无核、优质、不同熟期等性状的新品种,充分发挥“细胞工程”这一传统生物技术在柑橘遗传改良中的独特作用。重点内容包括 :1)细胞工程创新种质与新品种选育:体细胞胚/珠心胚发育机理,体细胞杂种、胞质杂种创制,离体培养与三倍体创制, 同源多倍体发掘与利用,纯系创制,细胞工程新品种选育与试验示范;2)细胞工程新种质特殊性状解析与分子育种:胞质杂种雄性不育性状解析与基因发掘,人工合成多倍体体细胞互作的遗传与表观遗传变异,转基因技术与分子育种。

代表性论著和成果 :

(* 通讯作者)

  • 1. Long JM, Liu CY, Feng MQ, Liu Y, Wu XM*, Guo WW* (2018) miR156-SPLs module regulates somatic embryogenesis induction in citrus callus. Journal of Experimental Botany, accepted
  • 2. Tan FQ, Tu H, Wang R, Wu XM, Xie KD, Chen JJ, Zhang HY, Xu J, Guo WW* (2017) Metabolic adaptation following genome doubling in citrus doubled diploids revealed by non-targeted metabolomics. Metabolomics 13: 143
  • 3. Long JM, Liu Z, Wu XM*, Fang YN, Jia HH, Xie ZZ, Deng XX, Guo WW* (2016) Genome-scale mRNA and small RNA transcriptomic insights into initiation of citrus apomixis. Journal of Experimental Botany 67: 5743–5756
  • 4. Fang YN, Zheng BB, Wang L, Yang W, Wu XM, Xu Q, Guo WW* (2016) High-throughput sequencing and degradome analysis reveal altered expression of miRNAs and their targets in a male-sterile cybrid pummelo (Citrus grandis). BMC Genomics 17:591
  • 5. Tan FQ, Tu H, Liang WJ, Long JM, Wu XM, Zhang HY, Guo WW* (2015) Comparative metabolic and transcriptional analysis of a doubled diploid and its diploid citrus rootstock (C. junos cv. Ziyang xiangcheng) suggests its potential value for stress resistance improvement. BMC Plant Biology 15: 89
  • 6. Wang SM, Lan H, Cao HB, Xu Q, Chen CL, Deng XX, Guo WW* (2015) Recovery and characterization of homozygous lines from two sweet orange cultivars via anther culture. Plant Cell Tissue Organ Culture 123:633-644
  • 7. Wu XM, Kou SJ, Liu YL, Fang YN, Xu Q, Guo WW* (2015) Genome-wide analysis of small RNAs in non-embryogenic and embryogenic tissues of citrus: microRNA and siRNA mediated transcript cleavage involved in somatic embryogenesis. Plant Biotechnology Journal 13: 383-394
  • 8. Xie KD, Xia QM, Wang XP, Liang WJ, Wu XM, Grosser JW, Guo WW* (2015) Cytogenetic and SSR marker evidence of mixed disomic, tetrasomic and intermediate inheritance in a citrus allotetraploid somatic hybrid between ‘Nova’ tangelo and ‘HB’ pummelo. Tree Genetics Genomes 11: 112
  • 9. Fang YN, Qiu WM, Wang Y, Wu XM, Xu Q, Guo WW* (2014) Identification of differentially expressed microRNAs from a male sterile Ponkan mandarin (Citrus reticulata Blanco) and its fertile wild type by small RNA and degradome sequencing. Tree Genetics Genomes 10: 1567-1581
  • 10. Xiao SX, Biswas MK, Li MY, Deng XX, Xu Q, Guo WW* (2014) Production and molecular characterization of diploid and tetraploid somatic cybrid plants between male sterile Satsuma mandarin and seedy sweet orange cultivars. Plant Cell Tissue Organ Culture 116: 81-88
  • 11. Xie KD, Wang XP, Biswas MK, Liang WJ, Xu Q, Grosser JW, Guo WW* (2014) 2n megagametophyte formed via SDR contributes to tetraploidization in polyembryonic ‘Nadorcott’ tangor crossed by citrus allotetraploids. Plant Cell Reports 33:1641–1650
  • 12. Zheng BB, Fang YN, Pan ZY, Sun L, Deng XX, Grosser JW, Guo WW* (2014) iTRAQ based quantitative proteomics analysis revealed alterations of carbohydrate metabolism pathways and mitochondrial proteins in a male sterile cybrid pummelo. Journal of Proteome Research 13: 2998-3015
  • 13. Ahmad T, Sablok G, Tatarinova T, Xu Q, Deng XX, Guo WW* (2013) Evaluation of codon biology in Citrus and Poncirus trifoliata based on genomic features and frame corrected expressed sequence tags. DNA Research 20: 135-150
  • 14. Guo WW*, Xiao SX, Deng XX (2013) Somatic cybrid production via protoplast fusion for citrus improvement. Scientia Horticulturae 163: 20-26
  • 15. Ge XX, Chai LJ, Liu Z, Wu XM, Deng XX, Guo WW* (2012) Transcriptional profiling of genes involved in embryogenic, non-embryogenic calluses and somatic embryogenesis of Valencia sweet orange by SSH based microarray. Planta 236: 1107-1124
  • 16. Kou SJ, Wu XM, Liu Z, Liu YL, Xu Q, Guo WW* (2012) Selection and validation of suitable reference genes for miRNA expression normalization by quantitative RT-PCR in citrus somatic embryogenic and adult tissues. Plant Cell Reports 31: 2151-2163
  • 17. Qiu WM, Zhu AD, Wang Y, Ge XX, Chai LJ, Deng XX, Guo WW* (2012) Comparative transcript profiling of gene expression between seedless Ponkan mandarin and its seedy wild type during floral organ development by suppression subtractive hybridization and cDNA microarray. BMC Genomics 13: 397
  • 18. Xu SX, Cai XD, Tan B, Li DL, Guo WW* (2011) Effect of ploidy increase on transgene expression: example from Citrus diploid cybrid and allotetraploid somatic hybrid expressing the EGFP gene. Protoplasma 248: 531-540
  • 19. Wu XM, Liu MY, Ge XX, Xu Q, Guo WW* (2011) Stage and tissue-specific modulation of ten conserved miRNAs and their targets during somatic embryogenesis of Valencia sweet orange. Planta 233: 495-505
  • 20. Tan B, Li DL, Xu SX, Fan GE, Fan J, Guo WW* (2009) Highly efficient transformation of GFP and KNU genes into precocious trifoliate orange (Poncirus trifoliata [L.] Raf), a potential model genotype for functional genomics studies in Citrus. Tree Genetics Genomes 5: 529-537
  • 21. Cai XD, Fu J, Deng XX, Guo WW* (2007) Production and molecular characterization of potential seedless cybrid plants between pollen sterile Satsuma and two seedy Citrus cultivars. Plant Cell Tissue Organ Culture 90: 275-283
  • 22. Guo WW*, Grosser JW* (2005) Somatic hybrid vigor in Citrus: direct evidence from protoplast fusion of an embryogenic callus line with a transgenic mesophyll parent expressing the GFP gene. Plant Science 168: 1541-1545
  • 23. Guo WW, Duan YX, Olivares-Fuster O, Wu ZC, Arias CR, Burns JK, Grosser JW (2005) Protoplast transformation and regeneration of transgenic Valencia sweet orange plants containing a juice quality related pectin methylesterase gene. Plant Cell Reports 24: 482-486
  • 24. Guo WW, Prasad D, Cheng YJ, Serrano P, Deng XX, Grosser JW (2004) Targeted cybridization in Citrus: transfer of Satsuma cytoplasm to seedy cultivars for potential seedlessness. Plant Cell Reports 22: 752-758
  • 25. Guo WW, Cheng YJ, Deng XX (2002) Regeneration and molecular characterization of intergeneric somatic hybrids between Citrus reticulata and Poncirus trifoliata. Plant Cell Reports 20: 829-834
  • 26. Guo WW, Deng XX (1999) Intertribal hexaploid hybrid plants regeneration from electrofusion between diploids of Citrus sinensis and its sexually incompatible relative, Clausena lansium. Theor Appl Genet 98: 581-585
  • 27. Guo WW, Deng XX (1998) Somatic hybrid plantlets regeneration between Citrus and its wild relative, Murraya paniculata via protoplast electrofusion. Plant Cell Reports 18: 297-300
  • 28. 梁武军,解凯东,郭大勇,谢宗周,徐强,伊华林,郭文武*(2014)柑橘10个品种实生后代多倍体的发掘与SSR鉴定。园艺学报 41:409-416
  • 29. 解凯东,王晓培,王惠芹,梁武军,谢宗周,郭大勇,伊华林,邓秀新,Grosser JW,郭文武*(2014)以柑橘多胚性二倍体母本倍性杂交培育三倍体。园艺学报 41:613-620
  • 30. 解凯东,王惠芹,王晓培,梁武军,谢宗周,伊华林,邓秀新,Grosser JW,郭文武*(2013)单胚性二倍体为母本与异源四倍体杂交大规模创制柑橘三倍体。中国农业科学 46:4550-4557
  • 31. 农业部植物新品种权(2015)。品种权名:华柚2号;品种权号:CNA20101034.2;品种权人:华中农业大学;培育人:郭文武、程运江、谢宗周、伊华林、邓秀新
  • 32. 授权国家发明专利(2016)。专利名称:一种早期筛选柑橘胞质杂种的方法;申请号:201310221676.9;发明人:郭文武、肖诗鑫